What is Protein A Resin? What are the Applications of Protein A Resin?

 

Protein A Resin
Protein A Resin

The mechanism for the degradation of Protein A resin particles that occurs during continuous chromatographic operation has been described using a modified shrinking core model (MSCM). The resin particles that contain the active Protein A ligands within their pores and serve as the basis of the model fictitiously shrink their boundary layer. It has been established that the caustic used in the sanitization stage of chromatography is what makes the Protein A ligand degrade. Manufacturer-provided Protein A resins have a special caustic stability that has been used in MSCM to assess ligand degradation. The diffusion constant, rate constant, stoichiometric factor, and reaction order were among the semiempirical parameters used in the kinetic model.

For three different resins, the parameters were calculated from column breakthrough experiments to simulate continuous Protein A Resin chromatography. The critical variable for forecasting the kinetics of degradation has been identified as the reaction order. Three different resins have different recorded reaction orders, with resin B having the highest order of 4 and resin C having the lowest order of 1.65. The model can forecast the effects of caustic on Protein A Resin performance and showed that, when exposed to 0.1 N and 0.2 N NaOH, the resins A and B underwent minimal degradation, maintaining up to 96% of their binding capacity after 240 cycles.

The degradation model was further supported by the adsorption study done on resin B, which showed the dynamic physical and chemical changes occurring throughout the resin's life cycle. According to the performance data, resin B performs as desired, with higher reaction order indicating slower resin deterioration, higher binding capacities, and increased sustenance of this binding capacity for longer periods of time. It is possible to develop efficient cleaning techniques for ongoing downstream processing by extending the degradation model.

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